نوع مقاله : مقاله پژوهشی
نویسندگان
1 استاد،دانشگاه علم و صنعت، تهران، ایران
2 دانشجوی دکتری،دانشگاه علم و صنعت ایران، تهران، ایران
3 استاد، دانشگاه تربیت مدرس، تهران، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The use of stirred tanks is very common in various industries due to their efficiency, proper mixing, and low cost. Usually, the stirrers used in these devices are impeller-type, which perform the mixing with a rotary movement. One of the most important problems of the impeller stirrers is the application of high shear stress, which is destructive in biological and sensitive applications. In this research, a novel laboratory-scale stirred reactor has been introduced, which provides mixing at low shear stress levels by using a perforated vibrating disc. Experimental investigation and numerical simulation of the stirred reactor have been done focusing on the effect of disc diameter. In the experimental section, Raji cell, as one of the most sensitive living particles, has been cultured in this stirred reactor using discs with a diameter of 25 and 65 (mm). Experimental results show the decreasing effect of disc diameter on cell growth indices, including the natural logarithm of maximum cell concentration and productivity. These indicators when using a 25 (mm) disc are high and about 13.2 and 4685 (cells/hmL), respectively, which indicates the proper performance of this stirred reactor in cell culture. The results of the numerical simulation show a tenfold increase in the energy dissipation rate factor with an increase in the disc diameter from 25 to 65 (mm), which indicates the logical agreement of the numerical simulation results with the experimental data.
کلیدواژهها [English]
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